z-logo
Premium
Phorbol Ester Activation of the Neuronal Nicotinic Acetylcholine Receptor α7 Subunit Gene
Author(s) -
CarrascoSerrano Carmen,
Viniegra Salvador,
Ballesta Juan J.,
Criado Manuel
Publication year - 2000
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.2000.0740932.x
Subject(s) - interleukin 10 receptor, alpha subunit , protein subunit , transfection , microbiology and biotechnology , acetylcholine receptor , nicotinic acetylcholine receptor , receptor , nicotinic agonist , interleukin 5 receptor alpha subunit , biology , phorbol , chemistry , transcription factor , gene expression , alpha 4 beta 2 nicotinic receptor , g alpha subunit , gene , biochemistry , signal transduction , protein kinase c
Abstract: α‐Bungarotoxin‐sensitive neuronal nicotinic acetylcholine receptors from bovine adrenomedullary chromaffin cells are up‐regulated by long‐term exposure to phorbol esters. The rise in receptor density is paralleled by an increase in transcripts corresponding to the α7 subunit, which is a component of this receptor subtype. Transcriptional activation of the α7 subunit gene is evidenced in reporter gene transfection experiments, in which phorbol esters increase α7 promoter activity by up to 14‐fold. About 80% of this activation is abolished when at least two of the three sites for the immediate‐early transcription factor Egr‐1, present in the proximal promoter region of the α7 subunit gene, are mutated simultaneously. In addition, phorbol esters elevate both Egr‐1 mRNA and Egr‐1 protein levels in chromaffin cells, whereas electrophoretic mobility shift assays show that the Egr‐1 component of the complexes that originate at the α7 promoter increases in cells treated with phorbol esters. These results suggest that the transcription factor Egr‐1 is involved in triggering expression of α‐bungarotoxin‐sensitive nicotinic receptors in response to external stimuli, such as the ones resulting from phorbol ester treatment, and support our previous hypothesis that the α7 subunit gene is one of the specific targets for Egr‐1.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here