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Role of Serotonin 2A and Serotonin 2B/2C Receptor Subtypes in the Control of Accumbal and Striatal Dopamine Release Elicited In Vivo by Dorsal Raphe Nucleus Electrical Stimulation
Author(s) -
De Deurwaerdère Philippe,
Spampinato Umberto
Publication year - 1999
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1999.0731033.x
Subject(s) - dorsal raphe nucleus , striatum , microdialysis , ritanserin , serotonin , stimulation , dopamine , chemistry , nucleus accumbens , raphe nuclei , medicine , 5 ht receptor , endocrinology , receptor , biology , serotonergic , biochemistry
: This study investigates, using in vivo microdialysis, the role of serotonin 2A (5‐HT 2A ) and 5‐HT 2B/2C receptors in the effect of dorsal raphe nucleus (DRN) electrical stimulation on dopamine (DA), 3,4‐dihydroxyphenylacetic acid (DOPAC), and 5‐hydroxyindoleacetic acid (5‐HIAA) extracellular levels monitored in the nucleus accumbens (NAC) and the striatum of halothane‐anesthetized rats. Following DRN stimulation (300 μA, 1 ms, 20 Hz, 15 min) DA release was enhanced in the NAC and reduced in the striatum. The 5‐HT 2A antagonist SR 46349B (0.5 mg/kg) and the mixed 5‐HT 2A/2B/2C antagonist ritanserin (0.63 mg/kg) significantly reduced the effect of DRN stimulation on DA release in the NAC but not in the striatum. DA responses to DRN stimulation were not affected by the 5‐HT 2B/2C antagonist SB 206553 (5 mg/kg) in either region. None of these compounds was able to modify the enhancement of DOPAC and 5‐HIAA outflow induced by DRN stimulation in either the NAC or the striatum. Finally, in both brain regions basal DA release was significantly increased only by SB 206553. These results indicate that 5‐HT 2A but not 5‐HT 2B/2C receptors participate in the facilitatory control exerted by endogenous 5‐HT on accumbal DA release. Conversely, 5‐HT 2B/2C receptors tonically inhibit basal DA release in both brain regions.