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Estrogen Hormones Reduce Lipid Peroxidation in Cells and Tissues of the Central Nervous System
Author(s) -
Vedder H.,
Anthes N.,
Stumm G.,
Würz C.,
Behl C.,
Krieg J.C.
Publication year - 1999
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1999.0722531.x
Subject(s) - endocrinology , medicine , lipid peroxidation , estrone , estrogen , incubation , hormone , chemistry , corticosterone , hippocampal formation , testosterone (patch) , steroid , biology , biochemistry , oxidative stress
Effects of estrogen hormones on lipid peroxidation (LPO)were examined in rat brain homogenates (RBHs), hippocampal HT 22 cells, ratprimary neocortical cultures, and human brain homogenates (HBHs).Dose‐response curves indicated half‐maximal effective concentrations(EC 50 ) of 5.5 and 5.6 m M for iron‐induced LPO in RBHs andHT 22 homogenates. Incubation of living rat primary neocortical cultures withiron resulted in an EC 50 of 0.5 m M , whereas culturehomogenates showed an EC 50 of 1.2 m M . Estrogen hormonesreduced LPO in all systems: In RBHs, estrone inhibited iron‐induced LPO to74.1 ± 5.8% of control levels (17β‐estradiol: 71.3 ± 0.1%)at a concentration of 10 μ M . In hippocampal HT 22 cellhomogenates, levels of LPO were reduced to 74.8 ± 5.5% by estrone andto 47.8 ± 6.2% by 17β‐estradiol. In living neocortical cultures,17β‐estradiol decreased iron‐induced LPO to 79.2 ± 4.8% andincreased the survival of cultured neuronal cells. Of the other steroidcompounds tested (corticosterone, progesterone, testosterone), onlyprogesterone decreased LPO in HT 22 cell homogenates. In HBHs, LPO wasdose‐dependently increased by iron concentrations from 2.7 to 6.0 m M .Incubation with estrogens resulted in a dose‐dependent inhibition of LPO to53.89 ± 8.6% with 10 μ M 17β‐estradiol, whereas estrone failed to affect iron‐induced LPO to a significant extent. Nonestrogenic steroids, including hydrocortisol, did not show significant effects on LPO in HBHs.