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Bone Morphogenetic Protein‐2 Promotes Survival and Differentiation of Striatal GABAergic Neurons in the Absence of Glial Cell Proliferation
Author(s) -
Hattori Akira,
Katayama Masahiro,
Iwasaki Shoji,
Ishii Kazuhiro,
Tsujimoto Masafumi,
Kohno Michiaki
Publication year - 1999
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1999.0722264.x
Subject(s) - neurotrophic factors , basic fibroblast growth factor , biology , neurotrophin , microbiology and biotechnology , glial cell line derived neurotrophic factor , gabaergic , neurite , striatum , bone morphogenetic protein , growth factor , endocrinology , medicine , in vitro , receptor , inhibitory postsynaptic potential , biochemistry , dopamine , gene
We examined the potential neurotrophic effects of bone morphogenetic protein (BMP)‐2 on the survival and differentiation of neurons cultured from the rat developing striatum at embryonic day 16, a period during which the mRNAs for BMP‐2 and its receptor subunits (types IA, IB, and II) were detected. BMP‐2 exerted potent activity to promote the survival of striatal neurons and increased the number of surviving microtubule‐associated protein‐2‐positive cells by 2.4‐fold as compared with the control cultures after 4 days in vitro. Although basic fibroblast growth factor (bFGF) also showed relatively high activity to promote the survival of striatal neurons, transforming growth factor‐β1, ‐β2, and ‐β3, glial cell line‐derived neurotrophic factor, or brain‐derived neurotrophic factor promoted their survival weakly. Striatal neurons cultured in the presence of BMP‐2 or bFGF possessed extensive neurite outgrowths, the majority of which were GABA‐immunoreactive. Inhibition of glial cell proliferation by 5‐fluorodeoxyuridine did not affect the capacity of BMP‐2 to promote the survival of striatal GABAergic neurons. In contrast, the ability of bFGF to promote the survival of striatal neurons was inhibited significantly by the treatment of cells with 5‐fluorodeoxyuridine. All these results suggest that BMP‐2 exerts potent neurotrophic effects on the striatal GABAergic neurons in a glial cell‐independent manner.