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Cationic Lipids and Polymers Are Able to Enhance Adenoviral Infection of Cultured Mouse Myotubes
Author(s) -
Dodds E.,
Piper T. A.,
Murphy S. J.,
Dickson G.
Publication year - 1999
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1999.0722105.x
Subject(s) - polyethylenimine , transduction (biophysics) , lipofectamine , adenovirus infection , myogenesis , genetic enhancement , adenoviridae , viral vector , microbiology and biotechnology , biology , cationic polymerization , gene delivery , c2c12 , reporter gene , myocyte , chemistry , virology , biochemistry , virus , gene expression , transfection , vector (molecular biology) , gene , recombinant dna , organic chemistry
Adenovirus‐mediated gene transfer has been used to promote efficient expression of various reporter and therapeutic transgenes such as minidystrophin in skeletal muscle tissue. However, down‐regulation of the adenovirus internalisation receptors, α v β 3 and α v β 5 , in adult myofibres and in mature cultured myotubes makes them less susceptible to infection than neonatal muscle or cultured myoblasts. It has been reported elsewhere that adenoviral transduction of cells that are normally refractory to infection can be enhanced by complexing virus particles with cationic lipids or cationic polymers. In this study we describe increased levels of adenovirusmediated transduction of cultured C2C12 myotubes, when the vector is complexed with either of the cationic lipids Lipofectamine or 1,3‐dioleoyloxy‐2‐(6‐carboxyspermyl)propylamide (DOSPER) or the cationic polymer polyethylenimine. The presence of polycations allowed a smaller dose of adenovirus vector to be used to attain the same level of infection seen with adenovirus alone, which has important relevance to future in vivo studies. Electron microscopic analysis of adenovirus/polycation complexes showed large aggregates as opposed to single adenovirus particles in the absence of polycations. Finally, by complexing adenovirus particles with polycations, partial protection against the neutralising effect of adenovirus antiserum was observed.