Agonist‐Independent Internalization and Activity of a C‐Terminally Truncated Somatostatin Receptor Subtype 2(δ349)

: The rat somatostatin receptor subtype 2 (SSTR2) is rapidly internalized and phosphorylated in the presence of somatostatin 14 (SST14). Several C‐terminal deletion constructs of SSTR2 have been investigated for their ability to undergo agonist‐dependent internalization by using biochemical ligand binding assays and confocal microscopic analysis. Whereas mutant receptors lacking either 10 (δ359), 30 (δ339), or 44 (δ325) amino acid residues at the C terminus required SST14 for internalization, a construct lacking the last 20 amino acids (δ349) was detected mostly intracellularly and independently of the presence of the agonist. When internalization was blocked by sucrose, the δ349 receptor remained at the cell surface, strongly indicating that this mutant is internalized in an agonist‐independent fashion. An increased affinity for agonists as measured in membrane binding assays and a reduced level of forskolin‐stimulated cyclic AMP accumulation in human embryonic kidney cells expressing δ349 are properties that are characteristic of agonist‐independent receptor activity. δ349 is not phosphorylated detectably in the absence of agonist, demonstrating that phosphorylation per se is not a prerequisite for internalization of SSTR2. This observation is in line with data obtained for the δ325 mutant, which was internalized in an agonist‐dependent manner, but not phosphorylated in either the presence or absence of SST14. We conclude that truncation of the SSTR2 C terminus at position 349 leads to agonist‐independent, constitutive activity and internalization.