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Formation of N ‐Pyroglutamyl Peptides from N ‐Glu and N ‐Gln Precursors in Aplysia Neurons
Author(s) -
Garden Rebecca W.,
Moroz Tatiana P.,
Gleeson Juliann M.,
Floyd Philip D.,
Li Lingjun,
Rubakhin Stanislav S.,
Sweedler Jonathan V.
Publication year - 1999
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1999.0720676.x
Subject(s) - aplysia , peptide , neurite , in vivo , biochemistry , chemistry , biology , in vitro , neuroscience , microbiology and biotechnology
: Matrix‐assisted laser desorption/ionization withtime‐of‐flight mass spectrometry is used to examine the formation of N ‐pyroglutamate (pGlu) in single, identified neurons from Aplysia . Six pGlu peptides are identified in the R3‐14 and the R15neurons that result from in vivo processing of peptides containing either Gluor Gln at their respective N‐termini. Moreover, we show that Glu‐derived pGluis not a sample collection or measurement artifact. The pGlu peptides aredetected in isolated cell bodies, regenerated neurites in culture,interganglionic connective nerves, cell homogenates, and collected releasates.We also demonstrate that R3‐14 cells readily convert a synthetic N ‐Glu peptide to its pGlu analogue, indicating the presence of novel enzymatic activity.