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Expression and Localization of Brain Ankyrin Isoforms and Related Proteins During Early Developmental Stages of Rat Nervous System
Author(s) -
Kunimoto Manabu,
Adachi Tatsumi,
Ishido Masami
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71062585.x
Subject(s) - ankyrin , gene isoform , biology , microbiology and biotechnology , gap 43 protein , ankyrin repeat , nervous system , central nervous system , neuroscience , immunohistochemistry , biochemistry , immunology , gene
Expression and localization of two isoforms of brain ankyrin, 440‐ and 220‐kDa ankyrin B , were studied in the developing nervous system of the rat fetus. The 440‐kDa ankyrin B appeared on as early as embryonic day 13, and its level increased progressively toward the day of birth, which was similar to the expression pattern of growth‐associated protein (GAP)‐43, a well‐established axonal protein. On the other hand, 220‐kDa ankyrin B was expressed at a low level but constitutively throughout the latter prenatal period and was a major isoform even before embryonic day 14. Whereas the localization of 440‐kDa ankyrin B was essentially confined to the axons, judging from the similarity with that of GAP‐43, 220‐kDa ankyrin B showed a rather general distribution in neural tissue. The localization of L1, known as an ankyrin B ‐binding protein, was similar to that of 440‐kDa ankyrin B in the brain tissue, whereas it was similar to that of 220‐kDa ankyrin B in cultured neurons, suggesting that the interaction of L1 with brain ankyrins in neurons is affected by their environment.