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Acute Effects of Ethanol on Pharmacologically Isolated Kainate Receptors in Cerebellar Granule Neurons: Comparison with NMDA and AMPA Receptors
Author(s) -
Valenzuela C. Fernando,
Bhave Sanjiv,
Hoffman Paula,
Harris R. Adron
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71041777.x
Subject(s) - kainate receptor , ampa receptor , ionotropic effect , nmda receptor , glutamate receptor , chemistry , long term depression , receptor , cnqx , ethanol , pharmacology , neuroscience , biophysics , biochemistry , biology
Comparisons of acute ethanol's effects on individual members of the three major families of ionotropic glutamate receptors (kainate, AMPA, and NMDA) have been performed only with recombinant receptors. However, no study has compared the acute effects of ethanol on individual members of each one of these receptor families in the same neuron. We accomplished this task by using cultured cerebellar granule neurons and LY303070 (GYKI‐53784), a noncompetitive and selective AMPA receptor antagonist. Ethanol concentrations of 25, 50, 75, and 100 m M decreased the amplitude of pharmacologically isolated kainate‐activated currents by 3 ± 1, 9 ± 2, 14 ± 2, and 22 ± 3% (n = 8), respectively. The magnitude of the ethanol‐induced inhibition of nonselective kainate‐activated currents, i.e., in the absence of LY303070, and currents activated by submaximal AMPA concentrations was not significantly different from that obtained with isolated kainate currents. However, the magnitude of the ethanol‐induced inhibition of NMDA receptor‐activated currents was about twofold greater than that of kainate and/or AMPA receptors.