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Persistent Expression of Fas/FasL mRNA in the Mouse Hippocampus After a Single NMDA Injection
Author(s) -
Shin SongWoo,
Park JongWook,
Suh MinHo,
Suh SungIl,
Choe ByungKil
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71041773.x
Subject(s) - fas ligand , nmda receptor , hippocampus , biology , neuroscience , apoptosis , messenger rna , gene expression , microbiology and biotechnology , chemistry , programmed cell death , receptor , gene , biochemistry
Synaptic reorganization plays a very important role in brain adaptations to environmental stimuli, diseases, and aging processes. The NMDA model of excitotoxic injury was used to investigate the long‐term molecular changes in the surviving neural cells in the mouse hippocampus. We demonstrated that a single intraperitoneal injection of NMDA produces persistent expression of c‐ fos , c‐ jun , Fas , and Fas ligand ( FasL ) mRNA in the hippocampus for 5 months. To determine the cellular origin of those gene transcripts in our in vivo model, a glial cell line and primary fetal neuronal culture were used to investigate the inducibility of the c‐ fos , c‐ jun , Fas , and FasL mRNA by NMDA. Both c‐ fos and Fas mRNA expression was observed in the NMDA‐treated glial or neuronal cultures; however, c‐ jun and FasL mRNA was undetectable in this study. In our in vivo model, mossy fiber sprouting and apoptosis were also observed up to 40 days after the NMDA injection. Therefore, we hypothesize that the observed long‐term expression of c‐ fos , c‐ jun , Fas , and FasL mRNAs may reflect the ongoing synaptic reorganization.