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Ca 2+ ‐ and Mg 2+ ‐Modulated Lipolysis in Neonatal Rat Brain Slices Observed by One‐ and Two‐Dimensional NMR
Author(s) -
Gasparovic Charles,
Berghmans Kirsten
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71041727.x
Subject(s) - lipolysis , perfusion , chemistry , perfusion scanning , endocrinology , medicine , biochemistry , biology , adipose tissue
Superfused cortical brain slices from neonatal rats demonstrated large increases in levels of NMR‐detectable lipids after sample preparation and perfusion with standard artificial CSF. These increases were reduced by an average of 58% by perfusion with buffer with low (no added) Ca 2+ or by perfusion in Ca 2+ ‐free buffer. Perfusion with buffer with elevated MgSO 4 (10 mmol/L) reduced the lipid changes by 47%. A reduction of 88% was observed in samples perfused in buffer with both low Ca 2+ and high Mg 2+ , suggesting a role for Mg 2+ in reducing lipolysis distinct from its known ability to block Ca 2+ influx.