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Regulation of Oleoyl‐CoA Synthesis in the Peripheral Nervous System: Demonstration of a Link with Myelin Synthesis
Author(s) -
Garbay Bertrand,
BoironSargueil Françoise,
Shy Michael,
Chbihi Taibi,
Jiang Huiyuan,
Kamholz John,
Cassagne Claude
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71041719.x
Subject(s) - myelin , sciatic nerve , remyelination , peripheral nervous system , gene isoform , messenger rna , biology , mutant , western blot , microbiology and biotechnology , central nervous system , chemistry , medicine , anatomy , biochemistry , endocrinology , gene
We studied the regulation of oleic acid synthesis in the PNS. During mouse postnatal development, the proportion of 18:1 rises in the sciatic nerve from 17% at 5 days of age to 33% at 25 days. However, this rise does not occur in the dysmyelinating mutant mouse trembler. In normal mouse development, the total stearoyl‐CoA desaturase (SCD) activity measured in sciatic nerve homogenates is high during the first 3 weeks. Yet in trembler nerves, this SCD activity represents only 15% of normal values. Using the RT‐PCR technique, we demonstrate that the SCD2 isoform is predominantly expressed in the PNS. Northern blot analysis showed that the mRNA levels for SCD2 parallel those of other specific myelin proteins in both normal mouse and trembler mutant development. Similar experiments in a rat demyelination‐remyelination model confirmed that SCD2 mRNA levels are regulated in the PNS in a similar manner to myelin‐specific proteins.