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Dominant Expression of Rat Prostanoid DP Receptor mRNA in Leptomeninges, Inner Segments of Photoreceptor Cells, Iris Epithelium, and Ciliary Processes
Author(s) -
Gerashchenko Dmitry,
Beuckmann Carsten T.,
Kanaoka Yoshihide,
Eguchi Naomi,
Gordon William C.,
Urade Yoshihiro,
Bazan Nicolas G.,
Hayaishi Osamu
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71030937.x
Subject(s) - biology , in situ hybridization , leptomeninges , receptor , ciliary body , melatonin receptor , retinal pigment epithelium , iris (biosensor) , microbiology and biotechnology , messenger rna , retina , endocrinology , central nervous system , neuroscience , biochemistry , gene , computer security , computer science , biometrics
Prostaglandin (PG) D 2 is one of the major prostanoids in the mammalian brain and eye tissues. Its function is mediated by the prostanoid DP receptor, which is specific for PGD 2 among the various prostanoids. In this study, we cloned the full‐length cDNA for the rat DP receptor and used it for detection of DP receptor mRNA in various rat tissues. Northern blotting and RT‐PCR analyses revealed that this DP receptor was expressed most intensely in the eye tissues, moderately in the leptomeninges and oviduct, and weakly in the epididymis. The tissue distribution profile of the mRNA for the rat DP receptor is overlapped with those of hematopoietic and lipocalin‐type PGD synthases. Among rat eye tissues, the expression was the highest in the iris. In situ hybridization and in situ RT‐PCR revealed DP receptor mRNA to be localized in the epithelium of the iris and ciliary body and in photoreceptor cells of the retina, suggesting the involvement of the receptor in the physiological regulation of intraocular pressure and the vision process. In the brain, DP receptor mRNA was dominantly expressed in the leptomeninges and was not detected in the brain parenchyma including the ventral rostral forebrain, the surface area of which is reportedly involved in sleep induction by PGD 2 .