Premium
Retinoic Acid Regulates the Developmental Expression of Dopamine D 2 Receptor in Rat Striatal Primary Cultures
Author(s) -
Valdenaire Olivier,
MausMoatti Marion,
Vincent JeanDidier,
Mallet Jacques,
Vernier Philippe
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.71030929.x
Subject(s) - retinoic acid , retinoic acid receptor beta , endocrinology , medicine , retinoic acid receptor , dopaminergic , retinoid x receptor gamma , biology , thyroid hormone receptor , dopamine receptor d2 , retinoic acid receptor alpha , retinoic acid inducible orphan g protein coupled receptor , receptor , retinoic acid receptor gamma , hormone , dopamine , cell culture , biochemistry , genetics
The time course of D 2 receptor expression assessed by the levels of the corresponding binding sites and mRNA was studied in rat striatum during ontogenesis and in primary cultures of cells taken at embryonic day (E) 17 and postnatal day (P) 4. In the two experimental situations, the amount of D 2 receptor mRNA and number of binding sites increased regularly from E16 to P15, indicating that expression of D 2 receptors in striatal neurons occurs independently from a dopaminergic input. Incubation of striatal primary cultures with 10 −5 M retinoic acid significantly increased the level of D 2 receptor mRNA, whereas thyroid hormone, vitamin D 3 , and steroid hormones (estradiol, testosterone, and corticosterone) had no effect. The transcriptional activity of the rat D 2 receptor gene promoter region, which bears a retinoic acid‐responsive element, was increased by retinoic acid in transfected C6 glioma cells but not in transfected MMQ prolactin cells. Thyroid hormone and vitamin D 3 were not effective in either cell line. Finally, mutations of the putative retinoic acid‐responsive element inhibited the transcriptional effect of retinoic acid. These results suggest that retinoic acid is a key factor in regulation of the embryonic onset of the dopaminergic D 2 receptor.