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Defining Responsiveness of Avian Cochlear Neurons to Brain‐Derived Neurotrophic Factor and Nerve Growth Factor by HSV‐1‐Mediated Gene Transfer
Author(s) -
Garrido Juan José,
Alonso Maria Teresa,
Lim Filip,
Carnicero Estela,
Giraldez Fernando,
Schimmang Thomas
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70062336.x
Subject(s) - nerve growth factor , neurotrophin , neurite , tropomyosin receptor kinase a , neurotrophic factors , neuroscience , biology , neurotrophin 3 , low affinity nerve growth factor receptor , brain derived neurotrophic factor , cochlea , amplicon , microbiology and biotechnology , receptor , gene , genetics , polymerase chain reaction , in vitro
The importance of individual members of the neurotrophin gene family for avian inner ear development is not clearly defined. Here we address the role of two neurotrophins, brain‐derived neurotrophic factor (BDNF) and nerve growth factor (NGF), for innervation of the chicken cochlea. We have used defective herpes simplex virus type 1 (HSV‐1) vectors, or amplicons, to express these neurotrophins in dissociated cultures of cochlear neurons. HSV‐1‐mediated expression of BDNF promotes neuronal survival similar to the maximal level seen by exogenously added BDNF and exceeds its potency to produce neurite outgrowth. In contrast, cochlear neurons transduced with an amplicon producing bioactive NGF show no response. These results confirm BDNF as an important mediator of neurotrophin signaling inside avian cochlear neurons. However, these neurons can be rendered NGF‐responsive by transducing them with the high‐affinity receptor for NGF, TrkA. This study underlines the usefulness of amplicons to study and modify neurotrophin signaling inside neurons.