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A Novel Orphan G Protein‐Coupled Receptor Primarily Expressed in the Brain Is Localized on Human Chromosomal Band 2q21
Author(s) -
Bläsius Rainer,
Weber Ruthild G.,
Lichter Peter,
Ogilvie Adaling
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70041357.x
Subject(s) - open reading frame , complementary dna , cdna library , microbiology and biotechnology , biology , southern blot , alternative splicing , genomic library , polyadenylation , clone (java method) , northern blot , untranslated region , genetics , gene , genomic dna , exon , gene expression , messenger rna , peptide sequence
A human hippocampus cDNA library was screened with a probe obtained from degenerate RT‐PCR aimed at P2Y‐homologous sequences. A positive clone, designated hip4, was identified containing an open reading frame of 1,020 bp that had been previously detected in a published genomic clone called R12. Subsequent screening of a human fetal brain cDNA library yielded a splice variant with a 1,104‐bp open reading frame, which was named fb1. Both variants display the seven‐transmembrane topology that is typical for G protein‐coupled receptors. Probing a human multitissue northern blot revealed two brain‐specific transcripts of 2.3 and 6.3 kb, respectively. Northern blot analyses with specific fragments confirmed that the two transcripts are generated by alternative polyadenylation yielding two different 3′ untranslated regions. A genomic clone from the corresponding gene was isolated and mapped to human chromosomal band 2q21 by fluorescence in situ hybridization.