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Stimulus‐Specific Hierarchy of Enhancer Elements Within the Rat Prodynorphin Promoter
Author(s) -
Carrión Angel M.,
Mellström Britt,
Luckman Simon M.,
Naranjo Jose R.
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70030914.x
Subject(s) - dynorphin , stimulation , biology , stimulus (psychology) , neuroscience , endocrinology , microbiology and biotechnology , chemistry , medicine , genetics , opioid peptide , psychology , opioid , receptor , psychotherapist
Induction of the prodynorphin gene occurs in a tissue‐specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP‐responsive element 2 (DynCRE2), DynCRE3, and the noncanonical prodynorphin AP‐1 (ncDynAP‐1) regulatory sites control, in a coordinated manner, prodynorphin induction in the spinal cord after noxious stimulation, whereas prodynorphin up‐regulation in supraoptic neurons is regulated predominantly by the ncDynAP‐1. Conversely, prodynorphin transactivation in the ovaries upon gonadotrophin stimulation is controlled by DynCRE1 and DynCRE3. Our results support the idea that stimulus‐specific changes in nuclear proteins establish a functional hierarchy among regulatory sites in the prodynorphin promoter and provide further insight in the molecular mechanisms that govern prodynorphin gene regulation.