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Examination of Blood‐Brain Barrier Transferrin Receptor by Confocal Fluorescent Microscopy of Unfixed Isolated Rat Brain Capillaries
Author(s) -
Huwyler Jörg,
Pardridge William M.
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70020883.x
Subject(s) - confocal microscopy , confocal , transferrin receptor , blood–brain barrier , fluorescence microscope , endothelium , microscopy , transferrin , membrane , biophysics , microbiology and biotechnology , monoclonal antibody , receptor , biology , chemistry , fluorescence , pathology , antibody , biochemistry , central nervous system , neuroscience , immunology , medicine , endocrinology , physics , geometry , mathematics , quantum mechanics
Isolated rat brain capillaries were analyzed by confocal microscopy. Fluorescent immunoliposomes bearing the OX26 anti‐transferrin receptor monoclonal antibody were synthesized and incubated with freshly isolated unfixed microvessels to visualize binding to luminal and abluminal membranes of the endothelium. Intactness of the endothelial structure was demonstrated by computer‐aided reconstruction of a series of consecutive optical sections. These results indicate that analysis of unfixed brain capillaries by confocal microscopy offers the possibility of assigning the presence of membrane receptors to either the luminal or the basolateral plasma membrane domain.