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Heparin Oligosaccharides that Pass the Blood‐Brain Barrier Inhibit β‐Amyloid Precursor Protein Secretion and Heparin Binding to β‐Amyloid Peptide
Author(s) -
Leveugle Béatrice,
Ding Wanhong,
Laurence Fenart,
Dehouck MariePierre,
Scanameo Andrew,
Cecchelli Roméo,
Fillit Howard
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70020736.x
Subject(s) - heparin , chemistry , secretion , amyloid (mycology) , peptide , amyloid precursor protein , biochemistry , in vitro , glycosaminoglycan , pharmacology , alzheimer's disease , medicine , biology , disease , inorganic chemistry
We have previously demonstrated that full‐length heparin stimulates the synthesis and secretion of β‐amyloid precursor protein (APP) through an amyloidogenic pathway in neuroblastoma cells. In the present study, heparin was chemically depolymerized, and the effect of low‐molecular‐weight (LMW) heparin on APP secretion was investigated. In contrast to full‐length heparin, LMW heparin had no significant effect on APP secretion. However, LMW heparin fragments, especially heparin disaccharides, were able to inhibit efficiently the stimulatory effect of heparin on APP secretion. LMW heparin derivatives also inhibit the binding of heparin to the β‐amyloid peptide (1–28). Using an in vitro model, we further demonstrated the passage of LMW heparin derivatives through the blood‐brain barrier. This study suggests that LMW heparin derivatives or analogues may be effective as therapeutic agents to prevent or slow the process of amyloidogenesis in Alzheimer's disease.