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Effect of Acute Systemic Inflammatory Response and Cytokines on the Transcription of the Genes Encoding Cyclooxygenase Enzymes (COX‐1 and COX‐2) in the Rat Brain
Author(s) -
Lacroix Steve,
Rivest Serge
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70020452.x
Subject(s) - inflammation , choroid plexus , cyclooxygenase , biology , tumor necrosis factor alpha , microglia , proinflammatory cytokine , lipopolysaccharide , interleukin 6 , endocrinology , in situ hybridization , gene expression , medicine , immunology , central nervous system , gene , enzyme , biochemistry
The aim of this study was to investigate the influence of the acute‐phase response and the proinflammatory cytokines on the transcription of the genes encoding the limiting enzymes for the production of prostaglandins, cyclooxygenase (COX)‐1 and COX‐2, in the rat brain. The bacterial endotoxin lipopolysaccharide (intravenous and intraperitoneal) and turpentine (intramuscular) were used as different models of inflammation in adult male rats. Animals were also killed at various times after intravenous administration of interleukin‐1β, tumor necrosis factor‐α, and interleukin‐6, and mRNAs encoding COX‐1 and COX‐2 were assayed by in situ hybridization histochemistry. A profound transcriptional activation of the gene encoding COX‐2 was detected over blood vessels of the entire brain microvasculature, choroid plexus, and leptomeninges of lipopolysaccharide‐challenged rats. Injection of the endotoxin intravenously also increased COX‐2 gene expression within parvocellular division of the hypothalamic paraventricular nucleus. It is interesting that intramuscular turpentine injection stimulated transcription of COX‐2 along endothelium of brain capillaries, and the signal of this transcript paralleled the inflammation of the left hind limb. A robust COX‐2 mRNA signal was detected rapidly in the brain microvessels of interleukin‐1β‐injected rats, whereas tumor necrosis factor‐α administration caused a modest but significant induction of this transcript. In contrast, intravenous injection of interleukin‐6 did not alter genetic expression of COX‐2, and none of the above described models affected the synthesis of COX‐1 gene in the rat brain. These results indicate that specific cell populations, in particular vascular‐ and/or perivascular‐associated cells, are responsible for the central production of prostaglandins during systemic inflammation, and circulating interleukin‐1β is likely to be a potent mediator of this response.