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Continuous Monitoring of Extracellular Glucose Concentrations in the Striatum of Freely Moving Rats with an Implanted Glucose Biosensor
Author(s) -
Lowry John P.,
O'Neill Robert D.,
Boutelle Martyn G.,
Fillenz Marianne
Publication year - 1998
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1998.70010391.x
Subject(s) - microdialysis , biosensor , extracellular , veratridine , chemistry , glucose oxidase , striatum , extracellular fluid , basal (medicine) , dialysis tubing , medicine , endocrinology , chromatography , biophysics , biochemistry , biology , sodium , insulin , dopamine , organic chemistry , sodium channel , membrane
We have used a glucose oxidase‐based sensor implanted in the striatum of freely moving rats to determine the concentration of extracellular glucose in two distinct ways. With a modification of the zero net flux method, in which different concentrations of glucose are infused through a dialysis probe glued to the biosensor, we calculated the concentration at which there was no change in glucose current by regression analysis; this gave a concentration of 0.351 ± 0.016 m M . Calculating the concentration from the basal current and the in vitro calibration of the biosensor was not significantly different from this. The basal extracellular glucose concentration determined by either method remained constant over a period of several days. Infusion of 50 µ M veratridine through the adjacent dialysis probe caused a steep decrease in glucose current as soon as the drug reached the brain in contrast to the delayed fall (7.5 min) seen with microdialysis in previous experiments from this laboratory. These results demonstrate that this biosensor provides a direct, real‐time measure of the extracellular concentration of glucose.