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Ethanol Specifically Inhibits NMDA Receptors with Affinity for Ifenprodil in the Low Micromolar Range in Cultured Cerebellar Granule Cells
Author(s) -
Engblom A. Christine,
Courtney Michael J.,
Kukkonen Jyrki P.,
Åkerman Karl E. O.
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.69052162.x
Subject(s) - ifenprodil , nmda receptor , chemistry , ethanol , potency , cerebellum , granule (geology) , glycine , antagonist , biochemistry , pharmacology , receptor , in vitro , biophysics , biology , endocrinology , amino acid , paleontology
The effect of ethanol on the intracellular Ca 2+ concentration response to NMDA in rat cerebellar granule cells grown in low or high KCI concentrations has been studied using image analysis. The cells grown in low KCI displayed high sensitivity for glycine. The subtype‐selective antagonist ifenprodil inhibited the response with high (in the low micromolar range) and low (in the high micromolar range) potency. Ethanol affected the high‐potency component in these cultures. In cells grown in high KCI the glycine sensitivity was lower, and a low potency for ifenprodil (high micromolar) dominated. These cells were not significantly sensitive to ethanol. The results indicate that the component displaying potency for ifenprodil in the low micromolar range with properties of the NR2B subunit is the target for ethanol action on the NMDA receptor.