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Identification of Kainate Receptor‐Mediated Intracellular Calcium Increases in Cultured Rat Cerebellar Granule Cells
Author(s) -
Savidge Jonathan R.,
Bleakman David,
Bristow David R.
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.69041763.x
Subject(s) - kainate receptor , ampa receptor , glutamate receptor , kainic acid , cnqx , biology , granule cell , chemistry , receptor , microbiology and biotechnology , neuroscience , biochemistry , hippocampal formation , dentate gyrus
The functional expression of the kainate subtype of glutamate receptor (GluR) has been investigated in cultured rat cerebellar granule cells using single cell intracellular calcium ([Ca 2+ ] i ) measurements. Both AMPA‐ and kainate‐induced [Ca 2+ ] i increases could be blocked completely by the AMPA receptor‐selective antagonist LY300168 (50 µ M ). However, following treatment with concanavalin A, an inhibitor of kainate receptor desensitisation, 30% of cells showed a kainate‐induced [Ca 2+ ] i rise of >100 n M in the presence of LY300168. Responses to 30 µ M kainate in the presence of LY300168 were virtually abolished by the AMPA and GluR5 kainate receptor competitive antagonist LY293558 (100 µ M ). These results demonstrate the presence of functional kainate receptors on cultured cerebellar granule cells, and suggest that the GluR5 subtype of kainate receptor plays a significant role in kainate receptor‐mediated [Ca 2+ ] i increases.