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Native Serotonin 5‐HT 3 Receptors Expressed in Xenopus Oocytes Differ from Homopentameric 5‐HT 3 Receptors
Author(s) -
Van Hooft Johannes A.,
Kreikamp Angélique P.,
Vijverberg Henk P. M.
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.69031318.x
Subject(s) - xenopus , receptor , serotonin , 5 ht receptor , dopamine , biology , dopamine receptor , microbiology and biotechnology , endocrinology , medicine , chemistry , biochemistry , gene
Abstract: Efficacies of the 5‐hydroxytryptamine (serotonin) 5‐HT 3 receptor (5‐HT 3 R) agonists 2‐methyl‐5‐HT, dopamine, and m ‐chlorophenylbiguanide on 5‐HT 3 R native to N1E‐115 cells and on homopentameric 5‐HT 3 R expressed in Xenopus oocytes were determined relative to that of 5‐HT. Efficacies of 2‐methyl‐5‐HT and dopamine on 5‐HT 3 R native to differentiated N1E‐115 cells are high (54 and 36%) as compared with their efficacies on homopentameric 5‐HT 3 R‐A L and 5‐HT 3 R‐A s receptors expressed in oocytes (4–8%). m ‐Chlorophenylbiguanide does not distinguish between 5‐HT 3 R in N1E‐115 cells and in oocytes. The distinct pharmacological profile of 5‐HT 3 R native to differentiated N1E‐115 cells is conserved when poly(A) + mRNA from these cells is expressed in oocytes. The results indicate that, apart from the known 5‐HT 3 R subunits, N1E‐115 cells express additional proteins involved in 5‐HT 3 R function.