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AP‐1 and Egr DNA‐Binding Activities Are Increased in Rat Brain During Ethanol Withdrawal
Author(s) -
Beckmann Alison M.,
Matsumoto Izuru,
Wilce Peter A.
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.69010306.x
Subject(s) - junb , fosb , cerebellum , hippocampus , transcription factor , striatum , cerebral cortex , dna , gene expression , microbiology and biotechnology , biology , cortex (anatomy) , chemistry , biochemistry , gene , endocrinology , neuroscience , dopamine
The DNA‐binding activities of AP‐1 and Egr proteins were investigated in nuclear extracts of rat brain regions during ethanol withdrawal. Both DNA‐binding activities were transiently elevated in the hippocampus and cerebellum 16 h after withdrawal. In the cerebral cortex, AP‐1 and Egr DNA‐binding activities increased at 16 h and persisted until 32 and 72 h, respectively. The AP‐1 DNA‐binding activities in all regions at all times after withdrawal were composed of FosB, c‐Jun, JunB, and JunD. c‐Fos was detected at all times in the cerebral cortex, at 16 h only in the hippocampus, and from 16 to 72 h in the cerebellum. Withdrawal severity did not affect the composition of the AP‐1 DNA‐binding activities. Two Egr DNA‐binding activities were present in the cortex and hippocampus. The faster‐migrating complex predominated in hippocampus, and only the slower‐migrating complex (identified as Egr‐1) was present in the cerebellum. The increase in DNA‐binding activity of immediate early gene‐encoded transcription factors supports their proposed role in initiating a cascade of altered gene expression underlying the long‐term neuronal response to ethanol withdrawal.