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Loss of the Xeroderma Pigmentosum Group A Gene ( XPA ) Enhances Apoptosis of Cultured Cerebellar Neurons Induced by UV but Not by Low‐K + Medium
Author(s) -
Enokido Yasushi,
Inamura Naoko,
Araki Toshiyuki,
Satoh Takumi,
Nakane Hironobu,
Yoshino Masafumi,
Nakatsu Yoshimichi,
Tanaka Kiyoji,
Hatanaka Hiroshi
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.69010246.x
Subject(s) - xeroderma pigmentosum , apoptosis , cerebellum , biology , microbiology and biotechnology , dna damage , programmed cell death , cancer research , dna , biochemistry , endocrinology
To study the involvement of the xeroderma pigmentosum group A gene ( XPA ) in neuronal apoptosis, we cultured cerebellar neurons from mice lacking XPA gene ( XPA −/− ) and induced apoptosis by exposure to UV irradiation or medium containing a low concentration of potassium (low‐K + medium). When cerebellar neurons from postnatal days 15–16 wild‐type mice were treated with UV irradiation, apoptotic neuronal death was observed after 24–48 h. About 60% of neurons survived 48 h after UV irradiation at a dose of 5 J/m 2 . On the other hand, neurons from XPA −/− mice showed a significantly increased vulnerability to UV irradiation, and >90% of neurons died 48 h after UV irradiation at a dose of 5 J/m 2 . In contrast, low‐K + medium induced apoptosis of neurons from mice of each genotype with the same kinetics. These results suggest that the XPA gene is involved in neuronal DNA repair and that it thereby influences apoptosis induced by DNA damage in cultured cerebellar neurons.