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Overexpression of Adhesion Molecule L1 in NG108‐15 Neuroblastoma × Glioma Hybrid Cells Enhances Dibutyryl Cyclic AMP‐Induced Neurite Outgrowth and Functional Synapse Formation with Myotubes
Author(s) -
Zhong ZhenGuo,
Yokoyama Shigeru,
Noda Mami,
Higashida Haruhiro
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.68062291.x
Subject(s) - neurite , transfection , microbiology and biotechnology , myogenesis , synapse , cell adhesion molecule , cell adhesion , biology , chemistry , cell culture , myocyte , cell , neuroscience , in vitro , biochemistry , genetics
The role of adhesion molecule L1 in synapse formation was examined by transient transfection of L1 cDNA in neuroblastoma × glioma hybrid NG108‐15 cells. L1 overexpression was found in ∼50% of the transfected NG108‐15 cell population. Neurite outgrowth induced by 0.25 m M dibutyryl cyclic AMP (cAMP) was much greater in L1‐transfected NG108‐15 cells than that in nontransfected and mock‐transfected cells. The proportion of cells with neurites and the number of neurites per cells were increased in L1‐transfected cells after 2 days of dibutyryl cAMP treatment. The proportion of cells with branched neurites and the average length of neurites were higher at day 4. A significantly higher rate of synapse formation with myotubes was apparent in the late phase of coculture (days 4–7) in L1‐transfected cells than in control cells. The miniature end‐plate potential frequency in myotubes was the same for the three types of NG108‐15 cells. These results show that overexpression of L1 in NG108‐15 cells facilitates synaptic connections by enhancing branching and elongation of neurites induced with dibutyryl cAMP, rather than by increasing probability of acetylcholine release.