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Expression of Krox Proteins During Differentiation of the O‐2A Progenitor Cell Line CG‐4
Author(s) -
Sock Elisabeth,
Leger Hubert,
Kuhlbrodt Kirsten,
Schreiber Jörg,
Enderich Janna,
RichterLandsberg Christiane,
Wegner Michael
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.68051911.x
Subject(s) - biology , cellular differentiation , microbiology and biotechnology , progenitor cell , oligodendrocyte , progenitor , myelin , immunology , stem cell , genetics , neuroscience , gene , central nervous system
Krox proteins are important regulators of development and terminal differentiation. Using the rat glial progenitor cell line CG‐4 as a model system for oligodendrocyte differentiation, we show that on the RNA level Krox‐24 is the predominant member of the Krox family in these cells. Similar results were also obtained on the protein level as the major Krox protein from CG‐4 cell extracts reacted specifically with an antibody against Krox‐24. Whereas Krox‐24 RNA and protein were abundant in undifferentiated CG‐4 cells, a dramatic decrease in expression was detected after a 3–5‐day period of differentiation during which we observed a reciprocal increase in the levels of myelin basic protein expression. Importantly, regulation of Krox‐24 expression was very similar in CG‐4 cells and primary oligodendrocyte cultures. When expression of Krox‐24 in differentiating CG‐4 cells was followed on a closer time scale, we observed a sharp and transient increase in Krox‐24 RNA, protein, and DNA binding activity immediately after the onset of differentiation followed by an equally rapid decrease. This expression pattern implicates Krox‐24 both in maintenance of the undifferentiated state and in the immediate early phase of differentiation of CG‐4 cells and possibly oligodendrocytes.