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Induction of a Distinct Morphology and Signal Transduction in TrkB/PC12 Cells by Nerve Growth Factor and Brain‐Derived Neurotrophic Factor
Author(s) -
Iwasaki Yasuno,
Ishikawa Mitsue,
Okada Noriko,
Koizumi Shinichi
Publication year - 1997
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1997.68030927.x
Subject(s) - nerve growth factor , neurotrophic factors , neurotrophin , brain derived neurotrophic factor , signal transduction , morphology (biology) , neuroscience , tropomyosin receptor kinase b , microbiology and biotechnology , growth factor , biology , chemistry , receptor , biochemistry , genetics
A clonal cell line stably expressing trkB (TrkB/PC12) was established from rat pheochromocytoma PC12 cells. Brain‐derived neurotrophic factor (BDNF), as well as nerve growth factor (NGF), stimulates neurite outgrowth in TrkB/PC12 cells. However, the morphology of BDNF‐differentiated cells was clearly different from NGF‐differentiated cells. BDNF treatment brought about longer and thicker neurites and induced a flattened soma and an increase in somatic size. This is not explained enough by the quantitative difference in the strength between TrkA and TrkB stimulation, because the level of BDNF‐stimulated tyrosine phosphorylation of TrkB was similar to that of TrkA stimulated with NGF in PC12/TrkB cells. There was no difference in major tyrosine phosphorylated proteins induced by NGF and BDNF. Signal proteins such as phosphatidylinositol 3‐kinase, phospholipase C‐γ1, Shc, and mitogen‐activated protein kinase seem to be involved in both TrkA‐ and TrkB‐mediated signaling pathways. However, a tyrosine‐phosphorylated 38‐kDa protein (pp38) was detected in anti‐pan‐Trk immunoprecipitation only after NGF stimulation. Immunoprecipitation using three distinct anti‐pan‐Trk antibodies suggests that pp38 is not a fragment of TrkA. These data indicate that TrkA has a unique signal transduction pathway that is not stimulated through TrkB in TrkB/PC12 cells and suggest distinct functions among neurotrophin receptors.