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A Bioluminescent γ‐Aminobutyrate Assay for Monitoring Its Release from Inhibitory Nerve Endings
Author(s) -
Israël M.,
Lesbats B.
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.67062624.x
Subject(s) - bioluminescence , photobacterium , luciferase , inhibitory postsynaptic potential , chemistry , succinic dehydrogenase , free nerve ending , succinate dehydrogenase , biochemistry , enzyme , biology , neuroscience , anatomy , vibrio , bacteria , transfection , gene , genetics
A bioluminescent GABA assay is described. The principle of the procedure is based on the action of GABASE (GABA‐aminotransferase plus succinic semialdehyde dehydrogenase), coupled to the detection of succinic semialdehyde and NADH, using Photobacterium luciferase. The method was used for monitoring GABA release from depolarized brain slices.