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Adenosine A 1 Receptors in Cultured Cerebellar Granule Cells: Role of Endogenous Adenosine
Author(s) -
Sanz Juana M.,
Vendite Deusa,
Fernández Mercedes,
Andrés Antonio,
Ros Manuel
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.67041469.x
Subject(s) - adenosine , adenosine a1 receptor , forskolin , medicine , adenosine deaminase , adenylate kinase , endocrinology , adenosine receptor , biology , receptor , purinergic signalling , cyclase , biochemistry , agonist
Adenosine A 1 receptors as well as other components of the adenylate cyclase system have been studied in cultured cerebellar granule cells. No significant changes in adenosine A 1 receptor number, assayed by radioligand binding in intact cells, were detected from 2 days in vitro (DIV) until 7 DIV. Nevertheless, a decline in this parameter was detected at 9 DIV. The steady‐state levels of α‐G s and α‐G i , detected by immunoblotting, showed similar profiles, increasing from 2 to 5 DIV and decreasing afterward. Forskolin‐stimulated adenylate cyclase levels also showed an increase until 5 DIV, decreasing at 7 and 9 DIV. The adenosine A 1 receptor analogue cyclopentyladenosine (CPA) was able to inhibit cyclic AMP accumulation at 2, 5, and 7 DIV but failed to do so at 9 DIV. This inhibition was prevented by the specific adenosine A 1 receptor antagonist 8‐cyclopentyl‐1,3‐dipropylxanthine. The presence of adenosine deaminase in the culture increased adenosine A 1 receptor number during the period studied and induced recovery of the inhibitory effect of CPA, lost after 7 DIV. These data suggest that functional expression of adenosine A 1 receptors and the other components of the adenylate cyclase system is subjected to regulation during the maturation of cultured cerebellar granule cells and demonstrates a key role for endogenous adenosine in the process.