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Three Dynamin‐Encoding Genes Are Differentially Expressed in Developing Rat Brain
Author(s) -
Cook T.,
Mesa K.,
Urrutia R.
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.67030927.x
Subject(s) - dynamin , gene isoform , gtpase , biology , gene , microbiology and biotechnology , messenger rna , gene expression , cdna library , genetics , receptor , endocytosis
Dynamin proteins are members of a recently described family of GTPases involved in receptor‐mediated processes. To date, three different dynamin‐encoding genes have been identified in mammalian tissues. Dynamin I is expressed only in neurons, whereas dynamin II is ubiquitously expressed. A third isoform, dynamin III, was originally isolated from a rat testis cDNA library and shown to be testis‐specific. However, here we report the cloning and characterization of dynamin III from brain and lung, demonstrating a more extended pattern of expression for this isoform. In addition, we have investigated the temporal pattern of expression of these three genes during brain development. We find that both dynamin I and dynamin III mRNA levels are up‐regulated during embryogenesis, whereas dynamin II mRNA levels remain unchanged. From these results, we conclude that dynamin III is not a testis‐specific isoform and, furthermore, that rat brain expresses three different dynamin‐encoding genes that are differentially regulated during development. Therefore, this large isoform diversity of dynamin proteins in brain predicts a significant complexity in the understanding of dynamin‐based processes in this tissue.