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Interaction of NMDA and Dopamine D 2L Receptors in Human Neuroblastoma SH‐SY5Y Cells
Author(s) -
Nair Venugopalan D.,
Niznik Hyman B.,
Mishra Ram K.
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.66062390.x
Subject(s) - receptor , dopamine , nmda receptor , neuroblastoma , sh sy5y , dopamine receptor , d1 like receptor , dopamine receptor d2 , dopamine receptor d3 , in vitro , chemistry , biology , microbiology and biotechnology , pharmacology , neuroscience , endocrinology , biochemistry , cell culture , genetics
To understand the mechanism of interaction of the dopamine D 2L receptors with NMDA receptors, we have developed a model by transfecting human neuroblastoma SH‐SY5Y cells with the human dopamine D 2L receptor gene. In vitro blockade of NMDA receptors by the specific antagonists MK‐801 and (±)‐3‐(2‐carboxypiperazin‐4‐yl)‐propyl‐1‐phosphonic acid (CPP) on human neuroblastoma SH‐SY5Y cells expressing human dopamine D 2L receptors resulted in a significant increase in the density of D 2L receptors without a significant change in receptor affinity. Moreover, the dopamine receptor mRNA level increased by ∼50% by the blockade of NMDA with MK‐801. These results suggest a possible interaction of NMDA and dopamine D 2L receptors in neuroblastoma SH‐SY5Y cells. This system would serve as an excellent model to study the molecular mechanisms involved in the interaction of these two receptors.