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Early Events in Free Radical‐Mediated Damage of Isolated Nerve Terminals: Effects of Peroxides on Membrane Potential and Intracellular Na + and Ca 2+ Concentrations
Author(s) -
Tretter László,
AdamVizi Vera
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.66052057.x
Subject(s) - cumene hydroperoxide , chemistry , intracellular , membrane potential , biophysics , depolarization , glutamate receptor , incubation , lipid peroxidation , biochemistry , oxidative stress , biology , receptor , catalysis
The effects of peroxides were investigated on the membrane potential, intracellular Na + ([Na + ] i ) and intracellular Ca 2+ ([Ca 2+ ] i ) concentrations, and basal glutamate release of synaptosomes. Both H 2 O 2 and the organic cumene hydroperoxide produced a slow and continuous depolarization, parallel to an increase of [Na + ] i over an incubation period of 15 min. A steady rise of the [Ca 2+ ] i due to peroxides was also observed that was external Ca 2+ dependent and detected only at an inwardly directed Ca 2+ gradient of the plasma membrane. These changes did not correlate with lipid peroxidation, which was elicited by cumene hydroperoxide but not by H 2 O 2 . Resting release of glutamate remained unchanged during the first 15 min of incubation in the presence of peroxides. These alterations may indicate early dysfunctions in the sequence of events occurring in the nerve terminals in response to oxidative stress.