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Protein Kinases Are Involved in Prolonged Acetylcholine Release from Rat Hippocampus Induced by Thyrotropin‐Releasing Hormone Analogue NS‐3
Author(s) -
Oka Michiko,
Itoh Yoshinori,
Ukai Yojiro,
Yoshikuni Yoshiaki,
Kimura Kiyoshi
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.66051889.x
Subject(s) - diagonal band of broca , acetylcholine , calphostin c , endocrinology , medicine , microdialysis , chemistry , hippocampal formation , thyrotropin releasing hormone , hippocampus , kinase , protein kinase c , biology , biochemistry , hormone , cholinergic neuron , central nervous system
The effects of various protein kinase inhibitors on acetylcholine release from the rat hippocampus induced by the local application of NS‐3 (montirelin hydrate, CG‐3703), a thyrotropin‐releasing hormone analogue, into the medial septum‐diagonal band were examined using in vivo microdialysis. Perfusion of NS‐3 (1 µ M ) into the medial septum‐diagonal band for 20 min produced a pronounced and prolonged increase in the hippocampal acetylcholine efflux. Pretreatment of the medial septum‐diagonal band with either K‐252a, a nonselective protein kinase inhibitor, or selective protein kinase A inhibitor H‐89 almost completely blocked the acetylcholine efflux evoked by NS‐3, and selective protein kinase C inhibitor calphostin C inhibited the action of NS‐3. On the other hand, NS‐3 (0.1–10 µ M ) or TRH (1–100 µ M ) increased the cyclic AMP efflux from the medial septum‐diagonal band in a concentration‐dependent manner, as measured by microdialysis. These findings suggest that protein kinases A and C in the neurons of the medial septum‐diagonal band are involved in the mechanism of the prolonged stimulation of acetylcholine release from the hippocampus induced by thyrotropin‐releasing hormone and its analogue, NS‐3.