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Expression of the β‐Galactoside α1,2‐Fucosyltransferase Gene Suppresses Axonal Outgrowth of Neuro2a Neuroblastoma Cells
Author(s) -
Hitoshi Seiji,
Kojima Naoya,
Kusunoki Susumu,
Inokuchi Jinichi,
Kanazawa Ichiro,
Tsuji Shuichi
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.66041633.x
Subject(s) - fucosyltransferase , neurite , ganglioside , cell culture , biology , microbiology and biotechnology , fucose , gene expression , glycolipid , gene , biochemistry , glycoprotein , in vitro , genetics
The axonal outgrowth of cells of Neuro2a, a mouse neuroblastoma cell line, was suppressed on expression of the β‐galactoside α1,2‐fucosyltransferase (α1,2‐FT) gene. We recently cloned two types of rabbit α1,2‐FT, RFT‐I and RFT‐II. RFT‐I exhibits comparable kinetic properties and structural homology with human H gene α1,2‐FT, and RFT‐II shows comparable kinetic parameters with human Se gene α1,2‐FT. Neuro2a cells expressing RFT‐I (N2A‐RFT‐I) contained a large amount of fucosyl GM1 instead of GM1 and GD1a, major gangliosides in the parent Neuro2a cells, whereas Neuro2a cells expressing RFT‐II (N2A‐RFT‐II) showed a subtle change in the ganglioside pattern. N2A‐RFT‐II and parent Neuro2a cells showed axonal outgrowth in serum‐free medium on the exogenous addition of GM1, whereas N2A‐RFT‐I cells exhibited multiple neurite sprouts but not axonal outgrowth. This phenotype was fully recovered by N2A‐RFT‐I cells on the addition of d ‐ threo ‐1‐phenyl‐2‐decanoylamino‐3‐morpholino‐1‐propanol and α‐ l ‐fucosidase to the culture medium, which resulted in pronounced reduction of fucosyl GM1 expression. These results suggested that expression of H‐type α1,2‐FT, and subsequent incorporation of fucose into glycolipids and glycoproteins, especially the formation of fucosyl GM1, modifies the response of neuronal cells to stimuli that induce axonal extension.