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In Vivo Expression of Inducible Nitric Oxide Synthase in Cerebellar Neurons
Author(s) -
MincGolomb Dahlia,
Yadid Gal,
Tsarfaty Ilan,
Resau James H.,
Schwartz Joan P.
Publication year - 1996
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1996.66041504.x
Subject(s) - nitric oxide synthase , in vivo , in situ hybridization , nitric oxide , biology , cerebellum , microbiology and biotechnology , lipopolysaccharide , immunohistochemistry , chemistry , gene expression , biochemistry , neuroscience , immunology , endocrinology , gene
In the CNS, nitric oxide (NO) functions as both neuromodulator and neurotoxic agent. In vivo neuronal expression of NO synthase (NOS) has been attributed to constitutive NOS—both the neuronal and the endothelial types. The other class of NOS—the inducible NOS (iNOS)—is known to mediate toxic effects of NO in various tissues. In this study, we show for the first time that direct intracerebellar injection of endotoxin and cytokine (lipopolysaccharide and interferon‐γ) induced in vivo neuronal expression of the iNOS gene, as demonstrated by fluorescent in situ hybridization and immunohistochemical staining analyzed by confocal laser‐scanning microscopy. This raises the possibility that neuronal iNOS might contribute significantly to the vulnerability of the brain to various insults.