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Effect of Reperfusion Following Cerebral Ischaemia on the Activity of the Mitochondrial Respiratory Chain in the Gerbil Brain
Author(s) -
Almeida Angeles,
Allen Kathryn L.,
Bates Timothy E.,
Clark John B.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.65041698.x
Subject(s) - gerbil , ischemia , respiration , respiratory system , respiratory chain , mitochondrion , nad+ kinase , cerebral ischaemia , mitochondrial respiratory chain , biochemistry , chemistry , biology , medicine , endocrinology , anesthesia , enzyme , anatomy
The effect of reperfusion following 30 min of cerebral ischaemia on brain mitochondrial respiratory chain activity has been studied in the gerbil. The state 3 respiration rates with both FAD‐ and NAD‐linked substrates were reduced after ischaemia. After 5 min of reperfusion, state 3 respiration with FAD‐linked substrates was restored, but levels of NAD‐linked substrates did not return to control values until 30 min of reperfusion. By 120 min of reperfusion state 3 respiration decreased relative to control values with all substrates studied. Measurement of the individual respiratory chain complexes showed that complex I, complex II–III, and complex V activities were reduced after ischaemia. By 5 min of reperfusion complex II–III activity was restored, but the activities of complexes I and V did not return to control values until 30 min of reperfusion. In contrast, complex IV activity was unaffected by ischaemia or 5 and 30 min of reperfusion but was significantly reduced after 120 min of reperfusion, possibly owing to free radical production and lipid peroxidation.