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Activation of Mitogen‐Activated Protein Kinase in Cultured Rat Hippocampal Neurons by Stimulation of Glutamate Receptors
Author(s) -
Kurino Masahito,
Fukunaga Kohji,
Ushio Yukitaka,
Miyamoto Eishichi
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.65031282.x
Subject(s) - metabotropic glutamate receptor , glutamate receptor , protein kinase c , mitogen activated protein kinase kinase , metabotropic glutamate receptor 6 , biology , metabotropic glutamate receptor 1 , map kinase kinase kinase , microbiology and biotechnology , mitogen activated protein kinase , nmda receptor , chemistry , biochemistry , kinase , receptor
Mitogen‐activated protein kinase (MAP kinase) was activated by stimulation of glutamate receptors in cultured rat hippocampal neurons. Ten micromolar glutamate maximally stimulated MAP kinase activity, which peaked during 10 min and decreased to the basal level within 30 min. Experiments using glutamate receptor agonists and antagonists revealed that glutamate stimulated MAP kinase through NMDA and metabotropic glutamate receptors but not through non‐NMDA receptors. Glutamate and its receptor agonists had no apparent effect on MAP kinase activation in cultured cortical astrocytes. Addition of calphostin C, a protein kinase C (PKC) inhibitor, or down‐regulation of PKC activity partly abolished the stimulatory effect by glutamate, but the MAP kinase activation by treatment with ionomycin, a Ca 2+ ionophore, remained intact. Lavendustin A, a tyrosine kinase inhibitor, was without effect. In experiments with 32 P‐labeled hippocampal neurons, MAP kinase activation by glutamate was associated with phosphorylation of the tyrosine residue located on MAP kinase. However, phosphorylation of Raf‐1, the c‐ raf protooncogene product, was not stimulated by treatment with glutamate. Our observations suggest that MAP kinase activation through glutamate receptors in hippocampal neurons is mediated by both the PKC‐dependent and the Ca 2+ ‐dependent pathways and that the activation of Raf‐1 is not involved.