Premium
Multiple Levels for Regulation of TrkA in PC12 Cells by Nerve Growth Factor
Author(s) -
Zhou Jie,
Valletta Janice S.,
Grimes Mark L.,
Mobley William C.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.65031146.x
Subject(s) - tropomyosin receptor kinase a , nerve growth factor , low affinity nerve growth factor receptor , biology , receptor tyrosine kinase , microbiology and biotechnology , tyrosine kinase , endocrinology , medicine , signal transduction , receptor , chemistry , biochemistry
TrkA is a receptor tyrosine kinase for nerve growth factor (NGF). Recent studies indicate that NGF regulates not only activation of trkA kinase but also expression of the trkA gene. To further define NGF actions on trkA, we examined binding and signaling through trkA after both short and long intervals of NGF treatment. Induction of tyrosine phosphorylation on gp140 trkA was rapidly followed by down‐regulation of cell surface and total cellular gp140 trkA . At later intervals, increased expression of trkA was evident in increased mRNA and protein levels. At 7 days, there was increased binding to gp140 trkA and increased signaling through this receptor. NGF appears to regulate trkA at several levels. In neurons persistently exposed to NGF, maintenance of NGF signaling may require increased trkA gene expression.