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Nitric Oxide‐Induced [ 3 H]GABA Release from Cerebral Cortical Neurons Is Mediated by Peroxynitrite
Author(s) -
Ohkuma Seitaro,
Narihara Hidehiko,
Katsura Masashi,
Hasegawa Takeshi,
Kuriyama Kinya
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.65031109.x
Subject(s) - peroxynitrite , superoxide , nitric oxide , sodium nitroprusside , chemistry , snap , superoxide dismutase , biophysics , biochemistry , gamma aminobutyric acid , biology , oxidative stress , enzyme , receptor , computer graphics (images) , organic chemistry , computer science
The functional significance of peroxynitrite in the release of [ 3 H]GABA induced by nitric oxide (NO) liberated from NO generators was investigated using cerebral cortical neurons in primary culture. NO generators such as sodium nitroprusside (SNP) and S ‐nitroso‐ N ‐acetylpenicillamine (SNAP) increased [ 3 H]GABA release in a dose‐dependent manner. These increases in [ 3 H]GABA release were significantly inhibited by hemoglobin, indicating that those NO generators evoke the release of [ 3 H]GABA by the formation of NO. Two types of superoxide scavengers, Cu 2+ /Zn 2+ superoxide dismutase and ceruloplasmin, significantly reduced the increase in [ 3 H]GABA release induced by both SNP and SNAP, which assumes that NO requires superoxide to induce [ 3 H]GABA release from the neurons. In addition, synthesized peroxynitrite induced a dose‐dependent increase in [ 3 H]GABA release from the neurons. These results indicate that NO‐induced [ 3 H]GABA release is mediated by peroxynitrite formed by the reaction of NO with superoxide.