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Evidence that 5′‐Cytidinediphosphocholine Can Affect Brain Phospholipid Composition by Increasing Choline and Cytidine Plasma Levels
Author(s) -
LópezCoviella Ignacio,
Agut Julian,
Savci Vahide,
Ortiz J. Alfonso,
Wurtman Richard J.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.65020889.x
Subject(s) - choline , phosphatidylcholine , phosphatidylethanolamine , phospholipid , phosphatidylserine , chemistry , endocrinology , medicine , oral administration , cytidine , dose–response relationship , biology , biochemistry , enzyme , membrane
We examined the effects of orally administered 5′‐cytidinediphosphocholine (CDP‐choline) on arterial plasma choline and cytidine levels and on brain phospholipid composition in rats. Animals receiving a single oral dose of 100, 250, or 500 mg/kg showed peak plasma choline levels 6–8 h after drug administration (from 12 ± 1 to 17 ± 2, 19 ± 2, and 24 ± 2 µ M , respectively). The area under the plasma choline curve at >14 µ M , i.e., at a concentration that induces a net influx of choline into the brain, was significantly correlated with CDP‐choline dose. In rats receiving 500 mg/kg this area was 2.3 times that of animals consuming 250 mg/kg, which in turn was 1.8 times that of rats receiving 100 mg/kg. Plasma cytidine concentrations increased 5.4, 6.5, and 15.1 times baseline levels, respectively, 8 h after each of the three doses. When the oral CDP‐choline treatment was prolonged for 42 and 90 days, brain phosphatidylcholine concentrations increased significantly (by 22–25%; p < 0.05) in rats consuming 500 mg/kg/day. Brain phosphatidylethanolamine and phosphatidylserine concentrations also increased significantly under some experimental conditions; levels of other phospholipids were unchanged.