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Sustained Dopamine Release Induced by Secretoneurin in the Striatum of the Rat: A Microdialysis Study
Author(s) -
Agneter E.,
Sitte H. H.,
StöcklHiesleitner S.,
FischerColbrie R.,
Winkler H.,
Singer E. A.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.65020622.x
Subject(s) - microdialysis , homovanillic acid , dopamine , 3,4 dihydroxyphenylacetic acid , striatum , medicine , endocrinology , chemistry , neurotransmitter , in vivo , neuropeptide , amino acid , biology , biochemistry , central nervous system , serotonin , receptor , microbiology and biotechnology
Secretoneurin (SN) is a neuropeptide derived from secretogranin II that is found in brain and endocrine tissues. The aim of the present study was to determine the influence of this novel peptide on dopamine (DA) release from rat striatum using the microdialysis technique. Rat SN (1–30 µmol/L added to the dialysis buffer) enhanced DA outflow of awake rats in a concentration‐dependent way without marked effects on the outflow of 3,4‐dihydroxyphenylacetic acid or homovanillic acid. The increase in extracellular DA content caused by the peptide was observed throughout the entire period of administration (up to 4 h). Human SN and its 15‐amino‐acid C‐terminal sequence also increased DA outflow, but the effects were smaller than those of rat SN. Two other peptides derived from secretogranin II were without effect on DA efflux. These results establish that SN has a pronounced effect on DA release under in vivo conditions.