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Laminin and Neuropeptide Y Are Increased by Synapsin Transfection in Cultured NG108‐15 Neuroblastoma/Glioma Hybrid Cells
Author(s) -
Fried Gabriel,
Han Hui Quan,
Meister Björn,
Hökfelt Tomas,
Greengard Paul
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64062674.x
Subject(s) - laminin , neuropeptide y receptor , transfection , neurite , biology , neuropeptide , cell culture , microbiology and biotechnology , neurotransmitter , chemistry , endocrinology , extracellular matrix , biochemistry , in vitro , central nervous system , genetics , receptor
We have investigated the presence and expression of laminin and neuropeptide Y (NPY) in several NG108‐15 cell lines transfected with synapsin Ib, IIa, or IIb. The content of laminin, a basal membrane glycoprotein that promotes adhesion and induces neurite outgrowth and neuronal differentiation, was increased in all transfected cell lines examined. In cells that were chemically differentiated with prostaglandin E 1 plus 3‐isobutyl‐1‐methylxanthine, laminin levels were increased even further. The content of NPY, suggested to be a neurotransmitter/neuromodulator in peripheral sympathetic neurons as well as in central neurons, was also increased in all transfected cell lines examined. Immunohistochemical analysis combined with confocal laser microscopy showed that NPY staining was granular and very often enriched in neuritic varicosities. The distribution and the staining pattern of NPY were consistent with storage of NPY in large dense‐cored vesicles. The results indicate that, in differentiated neurons, the synapsins increase the levels of a neuropeptide transmitter stored in large dense‐cored vesicles and of an extracellular matrix protein associated with neuronal maturation.