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Participation of Noradrenergic Neurotransmission in the Enhancement of Baroreceptor Reflex Response by Substance P at the Nucleus Tractus Solitarii of the Rat: A Reverse Microdialysis Study
Author(s) -
Chan Julie Y. H.,
Tsou MeiYung,
Len WenBin,
Lee TakYu,
Chan Samuel H. H.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64062644.x
Subject(s) - baroreceptor , microdialysis , solitary nucleus , nucleus , reflex , substance p , medicine , chemistry , neurotransmission , anesthesia , norepinephrine , endocrinology , neuroscience , stimulation , central nervous system , receptor , biology , heart rate , neuropeptide , dopamine , blood pressure
Abstract: We applied reverse microdialysis and HPLC analysis to evaluate the participation of noradrenergic neurotransmission in modulation of the baroreceptor reflex response by substance P at the nucleus tractus solitarii in Sprague‐Dawley rats anesthetized with pentobarbital sodium (50 mg/kg, i.p., with 20 mg/kg/h.i.v. supplement). Continuous infusion of substance P (600 µ M ) at 1 µl/min into the nucleus tractus solitarii through a stereo‐taxically positioned microdialysis probe (active exchange length, 180–200 µm; diameter, 220 µm) for 1 h elicited an enhancement of the baroreceptor reflex response. This facilitatory effect correlated positively, during the 60‐min infusion period, with the time course of increase in the extracellular concentration of substance P and noradrenaline in the nucleus tractus solitarii. Experimentally elevating the concentration of noradrenaline at this medullary nucleus also augmented the baroreceptor reflex sensitivity. On the other hand, depletion of the noradrenergic fibers and nerve terminals at the nucleus tractus solitarii with DSP4 diminished the enhancement of baroreceptor reflex response and the corresponding elevation in extracellular concentration of noradrenaline by substance P. Microinfusion of noradrenaline into the nucleus tractus solitarii in DSP4‐treated animals, however, potentiated the baroreceptor reflex response. These results suggest that the enhancement of baroreceptor reflex response by substance P may involve an increase in the concentration of noradrenaline at the nucleus tractus solitarii via a presynaptic mechanism.