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Activation of Muscarinic Receptors Inhibits Apoptosis in PC12M1 Cells
Author(s) -
Lindenboim Liora,
PinkasKramarski Ronit,
Sokolovsky Mordechai,
Stein Reuven
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64062491.x
Subject(s) - muscarinic acetylcholine receptor , oxotremorine , muscarinic acetylcholine receptor m1 , muscarinic acetylcholine receptor m4 , muscarinic acetylcholine receptor m3 , muscarinic acetylcholine receptor m2 , microbiology and biotechnology , muscarinic antagonist , apoptosis , biology , muscarinic acetylcholine receptor m5 , receptor , muscarinic agonist , chemistry , biochemistry
Previous studies have shown that PC12 cells depend on growth factors for their survival. When deprived of growth factors, the cells undergo a dying process termed “apoptosis” (programed cell death). We show here that muscarinic agonists inhibited the apoptotic death of growth factor‐deprived PC12M1 cells (PC12 cells stably expressing cloned m1 muscarinic acetylcholine receptors). This protective effect of the muscarinic agonists was observed in both proliferating and neuronal PC12M1 cells, was blocked by the muscarinic antagonist atropine, and was not observed in PC12 cells lacking m1 receptors. Muscarinic receptors therefore mediate inhibition of apoptosis in these cells. In addition to its effect on survival, the muscarinic agonist oxotremorine induced inhibition of DNA synthesis as well as growth arrest of exponentially growing PC12M1 cells at the S and G 2 /M phases of the cell cycle. Muscarinic receptors in these cells may therefore mediate inhibition of cell cycle progression.