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Nondestructive Measurement of Retinal Glucose Transport and Consumption In Vivo Using NMR Spectroscopy
Author(s) -
Berkowitz Bruce A.,
Garner Margaret H.,
Wilson Charles A.,
Corbett Ronald J. T.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64052325.x
Subject(s) - retinal , in vivo , chemistry , glucose transporter , carbohydrate metabolism , metabolism , retina , nuclear magnetic resonance spectroscopy , biophysics , biochemistry , endocrinology , biology , stereochemistry , neuroscience , microbiology and biotechnology , insulin
The cellular events underlying various retinopathies are poorly understood but likely involve perturbation of retinal glucose metabolism. Current methods for assessing this metabolism are destructive, thus limiting longitudinal studies. We hypothesize that following an intravitreous injection, the clearance rate of a glucose analogue will be a nondestructive index of retinal glucose transport and metabolism in vivo. First, radiolabeled glucose analogues were injected into the vitreous. After 40 min, the dominant clearance path was posterior via the retina and was consistent with a facilitated transport mechanism. Next, either [6,6‐ 2 H 2 ]glucose or 3‐deoxy‐3‐fluoro‐ d ‐glucose was injected into the vitreous of rabbit eyes, and the clearance rate of each analogue was determined over 40 min using, respectively, 2 H or 19 F NMR. These rates were interpreted as a function of the retinal glucose transport and consumption. From the NMR data, the rate of retinal glucose consumption was ∼16 times slower than the transport of glucose. These data demonstrate that NMR measurements of glucose analogue clearance rate from the vitreous can provide a nondestructive index of retinal glucose transport and consumption in vivo.