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Free Radical‐Generated Neurotoxicity of 6‐Hydroxydopamine
Author(s) -
Kumar Raj,
Agarwal Ashok K.,
Seth Prahlad K.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64041703.x
Subject(s) - chemistry , hydroxydopamine , endocrinology , medicine , glutathione peroxidase , lipid peroxidation , superoxide dismutase , phospholipid , glutathione , striatum , biochemistry , neurotoxicity , antioxidant , toxicity , membrane , biology , dopaminergic , dopamine , enzyme , organic chemistry
Albino rats were lesioned bilaterally with 6‐hydroxydopamine (6‐OHDA) hydrochloride (4 µg/µl, dissolved in saline with 0.1% ascorbic acid) into the striatum, and 72 h after the injection, levels of lipid peroxidation, GSH, superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), lipid class, membrane fluidity, and intracellular calcium concentrations were studied and the results were compared with those in the sham‐operated controls. The malonaldialdehyde level and the level of conjugated dienes were increased by 43 and 40%, respectively, in corpus striatum, and GSH, SOD, and GSH‐Px levels were decreased (24–30%) following 6‐OHDA treatment. Total phospholipid content was also decreased (18%), whereas cholesterol content remained unaffected. Among the different phospholipids only phosphatidylcholine and phosphatidylinositol were decreased in level. Membrane fluidity was decreased (23%), whereas the intracellular calcium concentration was elevated (100%) in corpus striatum compared with control rats. The results suggest that these alterations in membrane‐related events by 6‐OHDA could be due to free radical generation.