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Differentiation Effects of Ciliary Neurotrophic Factor on Human Neuroblastoma Cells
Author(s) -
Lawrance G.,
Rylett R. J.,
Richardson P. M.,
Dunn R. J.,
Dow K. E.,
Riopelle R. J.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64041483.x
Subject(s) - ciliary neurotrophic factor , choline acetyltransferase , neurite , leukemia inhibitory factor receptor , leukemia inhibitory factor , acetylcholinesterase , nerve growth factor , cholinergic , medicine , acetylcholine , cell culture , endocrinology , neuroblastoma , biology , neurotrophic factors , neurotrophin , ciliary ganglion , growth factor , choline , receptor , microbiology and biotechnology , cytokine , biochemistry , in vitro , interleukin 6 , immunology , enzyme , genetics
In the human neuroblastoma cell line LA‐N‐2, recombinant rat ciliary neurotrophic factor (CNTF) induced neurite growth and cholinergic differentiation that were both half‐maximally saturated at <100 p M of the neurokine, but was not required for cell survival in serum‐free conditions over a 13‐day period. CNTF markedly stimulated choline acetyltransferase activity and acetylcholine synthesis, whereas high‐affinity choline transport was only slightly enhanced and acetylcholinesterase activity was unchanged. Leukemia inhibitory factor had effects identical to CNTF on neurite growth and choline acetyltransferase activity, but interleukin 6 had no effect. Radioiodinated CNTF binding and affinity cross‐linking studies were consistent with tripartite receptor activation as a mediator of the observed biological effects.