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Effects of Non‐NMDA Receptor Modulators on [ 3 H]Dopamine Release from Rat Mesencephalic Cells in Primary Culture
Author(s) -
Petitet François,
Blanchard JeanCharles,
Doble Adam
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64031410.x
Subject(s) - nmda receptor , dopamine , neuroscience , chemistry , primary culture , dopamine receptor , midbrain , dopamine receptor d1 , receptor , pharmacology , biology , central nervous system , in vitro , biochemistry
The effects of AMPA and kainate on [ 3 H]dopamine release from fetal (embryonic day 15) rat mesencephalic neurons in primary culture were enhanced markedly in a dose‐dependent fashion by cyclothiazide, a recently described inhibitor of AMPA receptor desensitization. The EC 50 value for cyclothiazide was 2.2 ± 0.8 µ M . The release of [ 3 H]dopamine induced by both AMPA (or kainic acid) and the combination of AMPA (or kainic acid) with cyclothiazide was antagonized by specific antagonists like 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione or the noncompetitive benzodiazepine GYKI 52466. Unlike cyclothiazide, the lectin concanavalin A did not stimulate [ 3 H]dopamine release. These results established the involvement of AMPA‐preferring receptors on [ 3 H]dopamine release from rat mesencephalic neurons in primary culture and provided further evidence for the existence of regulatory allosteric sites on AMPA receptor subunits.